This project will be focused on the testing of the performance of a new type of parallel RF coil system that allows for significantly higher signal to noise and imaging performance than currently available coil systems (estimated 3 times higher signal to noise and acceleration of imaging times by a factor of 8-16 times). The […]

The intern will use screening methods to define which gene which are altered in the Jurkat human T cell line following incubation with the Verotoxin B subunit as a means to define the potential mechanism by which this treatment protects these cells from HIV1 infection in vitro. LISI Therapeutics will benefit by increasing their license […]

Vitamin D compounds are being developed for the treatment of chronic kidney disease. The purpose of this proposal is to develop a cell-based screening platform that will allow the rapid assessment ofrelative efficacy of a library of compounds. The intern will use recombinant DNA methodology to generate a cell-based assay in which the green fluorescence […]

We have discovered a set of naturally occurring GATA transcription factors that increase chlorophyll levels, chloroplast numbers and starch in plants. Maize (corn) and Oryza (rice) lines have been created by Syngenta and are being analyzed in the Rothstein laboratory. The project primarily involves determining the exact genes that are controlled by these transcription factors, […]

  In recent years, the interest given to disease biomarkers has boomed. Biotechnology and pharmaceutical companies are exploring ways to use biomarkers to speed up the drug development process, as well as to rapidly assess a diseases state, staging, progression and response to therapy. Multiple reaction monitoring (MRM) Mass Spectrometry (MS) has been shown to […]

The intern will be producing a therapeutic protein, i.e. ‘biologic’ (BMP2) using mutated bacterial plasmids as the bio-factory. The intern will create a sequence of DNA to be transformed into E. coli cells (BL21). The cells will be grown up in the lab, and studied for enhanced clinical efficacy. Proteins will be affinity purified and […]

Multiple antibiotic resistances have increased over the past decades, challenging our ability to treat bacterial infections and thwarting our ability to develop new antimicrobial agents. Many resistance genes have not evolved within the pathogenic isolates but were acquired by lateral transfer. We recently showed that genes conferring glycopeptide resistance are highly prevalent in the human […]

There is a pressing need to apply genomics-based tools for rapidly evaluating the impact of industrial and municipal waste management practices and climate change. In order to do this, information about the genome from relevant North American species is needed, yet acquiring these complex genome sequences had been cost and resource prohibitive until recently. Working […]

Type 2 diabetes is characterized by insulin resistance and has been associated with increased lipids (fat) in the blood (hyperlipidemia). Our research focuses on regulation of blood lipid levels by controlling both intestinal and liver fat (lipid/lipoprotein) metabolism. We have previously shown in our laboratory that a naturally occurring hormone called glucagon-like peptide-1 (GLP-1) can […]

Whether it is for research, development or diagnostics, life sciences typically require the quantitation and characterization of samples with assays that classically measure molecules one at a time. Considering that the development and execution of such assays are time consuming and requires considerable investment, the uses of multiplexed analysis would be highly advantageous. Mass spectrometry […]

We propose a meta-analytic project which aims at summarizing Post-Authorization Surveillance Safety data on Advate, a recombinant FVIII concentrates used to treat patients with Hemophilia A. The study will involve the largest cohort of hemophilia patients on FVIII concentrates analyzed in the literature. The project represents an attempt to sustain a methodologically strong approach in […]