Advanced differential mobility mass spectrometry with electron capture dissociation for the characterization of therapeutic proteins

Many new pharmaceuticals are based on large biomolecules like proteins. Even small differences in the protein structure can cause significant changes in the efficacy and safety of these drugs. Furthermore, these large molecules are difficult to characterize without advanced instrumentation and methods. Current technologies still struggle with robustness and reproducibility. This study aims to introduce new technology to improve the reliability of protein pharmaceutical characterization. It will leverage a separation technique called differential mobility spectrometry with an advanced mass spectrometry technique called electron capture dissociation to identify, characterize and verify the structure and shape of these therapeutics along with the presence of dangerous contaminants. These technologies will increase our ability to identify novel modifications much more quickly while simultaneously reducing our reliance on less robust liquid-based separation methods. Developing this application will provide the additional push needed to further commercialize and market both differential mobility spectrometry and electron capture dissociation.

Faculty Supervisor:

Derek Wilson

Student:

Brendon Seale

Partner:

AB SCIEX

Discipline:

Chemistry

Sector:

Pharmaceuticals

University:

Program: