Determination of stress-induced modifications to vaccine proteins by mass spectrometry

The therapeutic effect of a vaccine depends on its chemical composition, and in particular the integrity of its protein components. Conditions under which a vaccine is manufactured and stored can damage the proteins it contains; however, since proteins are structurally complex and reactive, their degradation during routine handling is unpredictable. In the project described here, three candidate vaccine products will be subjected to models of manufacture and storage in order to induce chemical changes within them in a controlled manner.

Adenovirus vaccination platform

Vaccine industry is evolving towards more efficient manufacturing systems. Adenovirus vectored vaccines are among new promising approaches for more efficient vaccines design and production. Recombinant Ad (rAd) can be used as gene deliver vector carrying different antigens for delivery into Ag-presenting cells (APC) eliciting a targeted immune response. Alternatively, antigens can be exposed on the rAd capsid, thus presenting the antigen to the immune system. Ad-vectored vaccines are safe, stable, have a broad tropism and have a large insert capacity and can be delivered orally.

Transdermal delivery of peptide hormones to treat tibial bone stress fractures using DiffusiMax liposomal cream base

The goal of this Mitacs research proposal study is to develop and apply experimental methodology for transdermal delivery of bone-targeting peptide hormone drugs. We aim to deliver these compounds using an appropriate hydrophilic-hydrophobic Diffusimax liposomal drug delivery cream base to anatomically superficial bone, at the sites of stress fractures (also known as “shin-splints”) in order to trigger bone repair, reduce pain burden, and to minimize possible side effects if those drugs were used systemically.

Production of bioactive peptides through high ydrostatic pressure assisted enzymatic digestion of egg white

Egg yolk contains IgY antibodies that target various infectious disease and food allergens. IGY Inc is a company focused on utilizing chicken immune system for antibody production. IgY antibody contains 0.64% of total wet egg yolk, after cracking each egg IGY Inc generates 60-70g of liquid egg white which has a low commercial value. Egg white is a rich source of dietary protein and also known for its biological activities. Therefore the primary objective of this study is to develop a pragmatic technique to hydrolyze egg white to produce peptides with biological activity.

Characterization of probiotics anti-apoptotic activity

Despite the high prevalence of Inflammatory Bowel diseases (IBD) in industrialized countries, particularly in Canada, the mechanisms controlling these debilitating gastrointestinal pathologies remain poorly understood. IBD is though to arise from an uncontrolled immune response to the intestinal microbiota, inducing a persistent inflammatory reaction in the gut. Death of epithelial cells and loss of epithelial barrier function are posited as key pathogenic events enabling bacterial translocation and the establishment of chronic inflammation.

Brain changes after 1 year of treatment with an injectable antipsychotic in people with a first-episode psychosis.

The proposed project will explore variations in the whole brain after 1 year of treatment that may exist between those who are taking an injectable antipsychotic versus those who are taking an oral antipsychotic in a cohort of first-episode of psychosis. All patients are treated in a naturalistic, early intervention setting at the Douglas Institute in Montreal, Canada. Janssen will benefit from the proposed project as it will help determine the benefits, if any, for prescribing an injectable earlier on in treatment,
namely related to brain tissue change.

Imaging Biomarkers for Nanotherapeutics

Poor drug accumulation at tumor sites negatively affects the efficacy of many anti-cancer therapeutics. Development of non-invasive strategies that can effectively monitor drug biodistribution is important, especially given the increasing number and complexity of nanotherapeutic agents in clinical development. The use of positron emission tomography in conjunction with a radiolabeled drug delivery system has the potential to achieve accelerated clinical translation. Merrimack Pharmaceuticals focuses on the development of nanotherapeutic platforms for cancer therapy and imaging.

Health economics and modeling collaboration between sanofi Pasteur and York: pertussis study

Pertussis, caused by the strict human pathogen B. pertussis or Bordetella parapertussis, is a highly contagious, potentially life-threatening respiratory tract illness when it occurs in unprotected infants. Despite the introduction of acellular pertussis (aP) vaccines and great initial success of the immunization program, pertussis outbreaks were reported in adolescents and adults in Canada since 1993. This trend was demonstrated as shift in pertussis epidemiology from infants and young children.

Data-driven approach to establish a design space for raw materials properties and manufacturing conditions for pharmaceutical drug stability assurance

Pharmaceutical companies are required to perform stability testing of their drug products by regulation. A drug product that succeeds on stability testing means that its identity, potency, and purity remain within pre-established limits over its shelf life. Nowadays, drug products are more frequently reformulated in response to a highly competitive market, new research discoveries and the availability of new suppliers of raw materials.

Tracking the fate of Tetrodotoxin bound sodium channels

Pain is a global clinical problem. Chronic pain caused by cancer, osteo/rheumatoid arthritis, operations, injuries and spinal problems affects millions of people worldwide. The current treatments for severe pain is unsatisfactory and include opioids, non-steroidal anti-inflammatory drugs, corticosteroids and tricyclic anti-depressants. Most of these drugs have limitations with regard to addiction, toxicity and thus there is a growing need for newer analgesic drugs that have greater efficacy, reduced toxicity and addiction.

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